DBS365BMP / in vitro differentiated cells
- Status: released
Summary
- Term name
- adipocyte
- Term ID
- CL:0000136
- Summary
- Homo sapiens adipocyte in vitro differentiated cells
- Description
- Primary bone-marrow derived human MSCs isolated from healthy donors. Adipogenic differentiation of the hMSCs was carried out 2 days after seeding using standard procedure for human cells. Briefly, cell monolayers were washed twice with phosphate buffered saline and supplemented with alpha-MEM consisting of 8.25% FBS, 0.5 µM water-soluble Dexamethasone, 0.5 µM isobutylmethylxanthine, and 50 µM Indomethacin. Alpha-MEM consisting of 8.25% FBS with or without supplementation were exchanged every 2–3 days until harvested
Attribution
- Lab
- Struan F.A. Grant, CHOP
- Award PI
- Struan F.A. Grant, CHOP
- Submitted by
- Ying Sun
- Source
- Unknown Source
- Project
- AMP
- Aliases
- struan-grant:biosample_13-124
- Submitter comment
- None post differentiation
Donor information
- Accession
- DDO848FFB
- Aliases
- struan-grant:donor_13-124
- Species
- Homo sapiens
- Sex
- unknown
- Health status
- Healthy
- Ethnicity
- Unknown