Summary for gene perturbation file set DSR220YNC
- Status: released
Summary
- Accession
- DSR220YNC
- Description
- Candidate gene screen for beta cell function using siRNA in EndoC-Bh1, cell number
- Biosample summary
- EndoC-BH1
- Biosample type
- cell line
- Biosample Conditions
- cell number
- Phenotypes Measured
- Insulin secretion, cell number
- Organism
- human
- Assay Design
- arrayed
- Assay Readout
- cellular phenotype
- Library
- custom
- Source of siRNA/shRNA
- Custom siRNAs Libraries (ON-TARGETplus SMARTpools [Dharmacon] & CyQUANT Direct Cell Proliferation kit (Thermo Fisher Scientific) on an EnVision plate reader (PerkinElmer)
- Commerical Assays
- AlphaLISA Human Insulin Immunoassays (PerkinElmer) on a PHERAstar FS plate reader (BMG Labtech) & CyQUANT Direct Cell Proliferation kit (Thermo Fisher Scientific) on an EnVision plate reader (PerkinElmer)
Attribution
AMP Consortium
- Lab
- AMP-T2D consortium
- Award
- U01DK100554 (T2D AMP Consortium)
- External resources
- Collection Tags
- None submitted
- Publications