Summary for gene perturbation file set DSR220YNC

  • Status: released

Summary

Accession
DSR220YNC
Description
Candidate gene screen for beta cell function using siRNA in EndoC-Bh1, cell number
Biosample summary
  • EndoC-BH1
(Homo sapiens)
Biosample type
  • cell line
Biosample Conditions
  • cell number
Phenotypes Measured
  • Insulin secretion, cell number
Organism
human
Assay Design
arrayed
Assay Readout
cellular phenotype
Library
custom
Source of siRNA/shRNA
Custom siRNAs Libraries (ON-TARGETplus SMARTpools [Dharmacon] & CyQUANT Direct Cell Proliferation kit (Thermo Fisher Scientific) on an EnVision plate reader (PerkinElmer)
Commerical Assays
AlphaLISA Human Insulin Immunoassays (PerkinElmer) on a PHERAstar FS plate reader (BMG Labtech) & CyQUANT Direct Cell Proliferation kit (Thermo Fisher Scientific) on an EnVision plate reader (PerkinElmer)

Attribution

AMP Consortium
Lab
AMP-T2D consortium
Award
U01DK100554 (T2D AMP Consortium)
External resources
Collection Tags
None submitted