Summary for gene perturbation file set DSR692EGA
- Status: released
Summary
- Accession
- DSR692EGA
- Description
- Genome-wide pooled CRISPR LoF screen for insulin content in EndoC-Bh1
- Biosample summary
- EndoC-BH1
- Biosample type
- cell line
- Biosample Conditions
- Phenotypes Measured
- insulin content
- Organism
- human
- Assay Design
- genome-wide pooled
- Assay Readout
- Sequencing of sorted samples
- Pooled Design
- sorting
- Library
- TKOv3
- Gating Strategy
- INSLOW and INSHIGH samples were gated based on live and single cells, low INS levels were determined based on EV control cells (INSLOW) and the highest 10 % of INS-expressing cells were sorted as high INS.
- Software used
- mageckv0.5.9.2
Attribution
AMP Consortium
- Lab
- AMP-T2D consortium
- Award
- U01DK100554 (T2D AMP Consortium)
- Aliases
- amp-t2d-consortium:AntjeKRottner_NatGenet2022_perturbations_DSR692EGA
- External resources
- https://hugeamp.org:8000/research.html?pageid=beta_cell_249
- Collection Tags
- CMDGA