Summary for gene perturbation file set DSR997MQA

  • Status: released

Summary

Accession
DSR997MQA
Description
CRISPR knockout of RREB1 expression in glucose-stimulated EndoC-BH1 cells after forskolin treatment (1 mmol/L glucose, 10 umol/L forskolin)
Biosample summary
  • EndoC-BH1
(Homo sapiens)
Biosample type
  • cell line
Biosample Conditions
  • 1 mmol/L glucose, 10 umol/L forskolin
Phenotypes Measured
  • Insulin secretion, insulin content
Organism
human
Assay Design
single gene
Assay Readout
Insulin ELISA
Library
custom
Commerical Assays
Insulin (human) AlphaLISA Detection Kit (PerkinElmer)
Pipeline used

Attribution

Community submission
Lab
Anna Gloyn, Oxford
Award
UM1DK126185 (Anna Gloyn, Oxford)
Aliases
  • anna-gloyn:CRISPR_KO_RREB1_1glucose-stimulatedEndoC-BH1_forskolinTreatment_perturbation
External resources
None submitted
Collection Tags
  • CMDGA