RNA Sequencing of Single Human Islet Cells Reveals Type 2 Diabetes Genes
Cell Metabolism. 2016-10-11;24(4):608-615.
- Abstract
- Pancreatic islet cells are critical for maintaining normal blood glucose levels, and their malfunction underlies diabetes development and progression. We used single-cell RNA sequencing to determine the transcriptomes of 1,492 human pancreatic α, β, δ, and PP cells from non-diabetic and type 2 diabetes organ donors. We identified cell-type-specific genes and pathways as well as 245 genes with disturbed expression in type 2 diabetes. Importantly, 92% of the genes have not previously been associated with islet cell function or growth. Comparison of gene profiles in mouse and human α and β cells revealed species-specific expression. All data are available for online browsing and download and will hopefully serve as a resource for the islet research community.
- Consortium data used in this publication
- GSE81608
- Datasets
- TSTSR426028, TSTSR857989, TSTSR890169, TSTSR217315, TSTSR173932, TSTSR549347, TSTSR123695, TSTSR545287