A chromosome folding intermediate at the condensin-to-cohesin transition during telophase
Nat Cell Biol. 2019-11-04;21(11):1393-1402.
- Abstract
- Chromosome folding is modulated as cells progress through the cell cycle. During mitosis, condensins fold chromosomes into helical loop arrays. In interphase, the cohesin complex generates loops and topologically associating domains (TADs), while a separate process of compartmentalization drives segregation of active and inactive chromatin. We used synchronized cell cultures to determine how the mitotic chromosome conformation transforms into the interphase state. Using high-throughput chromosome conformation capture (Hi-C) analysis, chromatin binding assays and immunofluorescence, we show that, by telophase, condensin-mediated loops are lost and a transient folding intermediate is formed that is devoid of most loops. By cytokinesis, cohesin-mediated CTCF-CTCF loops and the positions of TADs emerge. Compartment boundaries are also established early, but long-range compartmentalization is a slow process and proceeds for hours after cells enter G1. Our results reveal the kinetics and order of events by which the interphase chromosome state is formed and identify telophase as a critical transition between condensin- and cohesin-driven chromosome folding.
- Datasets
- DSR349TAH, DSR800RWP, DSR848YKD, DSR986KRG, DSR815JQD, DSR252HPT, DSR961OZM, DSR218AFF, DSR441OZK, DSR746OLK, DSR270QYA, DSR616RTF, DSR580WYQ, DSR407SCI, DSR233QGW, DSR953QKJ, DSR708PPR, DSR198IQX, DSR997TFK, DSR564ZNK, DSR959VCI, DSR697WYG, DSR108DZW, DSR873REM, DSR898CUV, DSR451EUM, DSR844NLS, DSR822QTR, DSR755LAJ, DSR589YJD, DSR312MTW, DSR980CAS, DSR692CHC, DSR101RDD, DSR304LFG, DSR508RSZ, DSR084LSY, DSR444PAE, DSR134HEV, DSR290YST, DSR250BAW, DSR584YJH, DSR730DRO, DSR349VSK, DSR669GDS, DSR230PJM, DSR854NBZ, DSR227CQV, DSR562BMX, DSR785KNF, DSR458ECQ, DSR946HKX, DSR634VBO, DSR691ZOE, DSR178ZZT, DSR378BWS, DSR651KNM, DSR447OYO, DSR921PXE, DSR188ZCT, DSR996GXV, DSR544NQI, DSR965UQX, DSR793VZA, DSR486NIF, DSR606IXF, DSR734TZE, DSR361QEH, DSR152ZTQ, DSR032GSR, DSR345MQU, DSR816CQC, DSR861OQE, DSR441OUJ, DSR440HNC, DSR684GVA, DSR008PVU, DSR953WKH, DSR736OCY, DSR306NMW, DSR401JCA, DSR473LFG, DSR144OPW, DSR440PKL, DSR399XDV, DSR727PII, DSR106JYI, DSR173LGI, DSR625XIC, DSR422CCE
Related data
- Data summary
- Sequencing data generated in this study have been deposited in the Gene Expression Omnibus (GEO) repository under accession GSE133462. Dataset titled “R1” refers to replicate 1 which is used in Figures 1--5,5, and Extended Data 1--5.5. Dataset titled “R2” refers to replicate 2 which is used in Figure 2, and Extended Data 1 and and6.6. Dataset titled “R3” refers to replicate 3 which is used in Figure 2, and Extended Data 1 and and7.7.
- Available data
- website
- Data summary
- Data from this publication can also be accessed in the 4DN Data Portal with link https://data.4dnucleome.org/abramo_et_al_2019. All other data supporting the findings of this study are available from the corresponding author on reasonable request.