Bivariate genome-wide association meta-analysis of pediatric musculoskeletal traits reveals pleiotropic effects at the SREBF1/TOM1L2 locus

Carolina Medina-Gomez, John P. Kemp, Niki L. Dimou, Eskil Kreiner, Alessandra Chesi, Babette S. Zemel, Klaus Bønnelykke, Cindy G. Boer, Tarunveer S. Ahluwalia, Hans Bisgaard, Evangelos Evangelou, Denise H. M. Heppe, Lynda F. Bonewald, Jeffrey P. Gorski, Mohsen Ghanbari, Serkalem Demissie, Gustavo Duque, Matthew T. Maurano, Douglas P. Kiel, Yi-Hsiang Hsu, Bram C.J. van der Eerden, Cheryl Ackert-Bicknell, Sjur Reppe, Kaare M. Gautvik, Truls Raastad, David Karasik, Jeroen van de Peppel, Vincent W. V. Jaddoe, André G. Uitterlinden, Jonathan H. Tobias, Struan F.A. Grant, Pantelis G. Bagos, David M. Evans & Fernando Rivadeneira.
Nature Communications. 2017-07-25;8(1):121.
Abstract
Bone mineral density is known to be a heritable, polygenic trait whereas genetic variants contributing to lean mass variation remain largely unknown. We estimated the shared SNP heritability and performed a bivariate GWAS meta-analysis of total-body lean mass (TB-LM) and total-body less head bone mineral density (TBLH-BMD) regions in 10,414 children. The estimated SNP heritability is 43% (95% CI: 34–52%) for TBLH-BMD, and 39% (95% CI: 30–48%) for TB-LM, with a shared genetic component of 43% (95% CI: 29–56%). We identify variants with pleiotropic effects in eight loci, including seven established bone mineral density loci: WNT4, GALNT3, MEPE, CPED1/WNT16, TNFSF11, RIN3, and PPP6R3/LRP5. Variants in the TOM1L2/SREBF1 locus exert opposing effects TB-LM and TBLH-BMD, and have a stronger association with the former trait. We show that SREBF1 is expressed in murine and human osteoblasts, as well as in human muscle tissue. This is the first bivariate GWAS meta-analysis to demonstrate genetic factors with pleiotropic effects on bone mineral density and lean mass.
Consortium data used in this publication
Expression data from bone biopsies is available at the European Bioinformatics Institute (EMBL-EBI) ArrayExpress repository, ID: E-MEXP-1618.
Datasets
DSR303SZB, DSR940ANL, DSR355SOP, DSR352DDN, DSR953TPX, DSR985FFO, DSR089BZF, DSR014BNH, DSR027CAY, DSR749HBK, DSR921GLP, DSR817ZLW, DSR181IDH, DSR274CSP, DSR646UAD, DSR285EAM, DSR678ANF, DSR915VED, DSR634THU, DSR952WEH, DSR798VOI, DSR964MIC, DSR918SLN, DSR924ABD, DSR219OPG, DSR661VWL, DSR278PLL, DSR796CIR, DSR907FIU, DSR921MMW, DSR824LTM, DSR086HDE, DSR799SHI, DSR515FBW, DSR997UXG, DSR663GZF, DSR087JQY, DSR004LZY, DSR154FSA, DSR608HLW, DSR554FGX, DSR996LRO, DSR993ZFI, DSR872WZU, DSR112LZL, DSR073WQU, DSR062FCS, DSR255XTN, DSR206AJK, DSR788NBJ, DSR018POB, DSR345HIM, DSR497FUW, DSR515CHF, DSR749YVJ, DSR472HQZ, DSR019LII, DSR159JXJ, DSR649HND, DSR658TCD, DSR697RKT, DSR248CMC, DSR093NAI, DSR776IJD, DSR486GDP, DSR405HLO, DSR152OQN, DSR175NVC, DSR494QZC, DSR320FNO, DSR920IMP, DSR231GPB, DSR759ERJ, DSR089CMY, DSR805GTS, DSR636QPR, DSR689JCC, DSR868NHW, DSR162NVO, DSR544PQB, DSR514DAO, DSR623ZLV, DSR051NCE, DSR338PVC